Respiratory syncytial virus-induced CCL5/RANTES contributes to exacerbation of allergic airway inflammation

Eur J Immunol. 2003 Jun;33(6):1677-85. doi: 10.1002/eji.200323930.


Severe respiratory syncytial virus (RSV) infection has a significant impact on airway function and may induce or exacerbate the response to a subsequent allergic challenge. In a murine model combining early RSV infection with later cockroach allergen (CRA) challenge, we examined the role of RSV-induced CCL5/RANTES production on allergic airway responses. RSV infection increased CCL5 mRNA and protein levels, peaking at days 8 and 12, respectively. Administration of CCL5 antiserum during days 0-14 of the RSV infection did not significantly alter viral protein expression when compared to mice treated with control serum. In mice receiving the combined RSV-allergen challenge, lungs collected on day 22 exhibited significantly increased numbers of CD4- and CD8-positive T cells. This increase in T cell numbers was not observed in mice receiving alpha-CCL5. On day 43, peribronchial eosinophilia and leukotriene levels were increased in RSV-allergen mice. Pretreatment with CCL5 antiserum resulted in decreased recruitment of inflammatory cells to bronchoalveolar and peribronchial regions of the lungs and these reductions were associated with a reduction in both T cell recruitment into the bronchoalveolar space, leukotriene release and chemokine generation. Thus, CCL5 released during RSV infection has a significant effect on the inflammatory response to subsequent allergic airway challenges.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Allergens / immunology
  • Animals
  • Asthma / etiology
  • Bronchi / immunology
  • Bronchi / pathology
  • Bronchoalveolar Lavage Fluid / immunology
  • CD4-Positive T-Lymphocytes / immunology
  • CD8-Positive T-Lymphocytes / immunology
  • Chemokine CCL5 / antagonists & inhibitors
  • Chemokine CCL5 / biosynthesis
  • Chemokine CCL5 / immunology
  • Chemokine CCL5 / physiology*
  • Chemokines / biosynthesis
  • Chemotaxis, Leukocyte
  • Cockroaches / immunology
  • Eosinophilia / etiology
  • Female
  • Flow Cytometry
  • Gene Expression Regulation, Viral
  • Humans
  • Immune Sera
  • Inflammation
  • Leukotrienes / metabolism
  • Lung / immunology
  • Lung / pathology
  • Mice
  • Mice, Inbred DBA
  • Models, Animal
  • Respiratory Hypersensitivity / etiology*
  • Respiratory Hypersensitivity / physiopathology
  • Respiratory Hypersensitivity / prevention & control
  • Respiratory Syncytial Virus Infections / complications
  • Respiratory Syncytial Virus Infections / immunology
  • Respiratory Syncytial Virus Infections / physiopathology*
  • Specific Pathogen-Free Organisms
  • Viral Proteins / biosynthesis


  • Allergens
  • Chemokine CCL5
  • Chemokines
  • Immune Sera
  • Leukotrienes
  • Viral Proteins