Abstract
A particulate subcellular fraction from Escherichia coli K-12 induced in anaerobic sn-glycerol 3-phosphate (G3P) dehydrogenase and fumarate reductase can catalyze under anaerobic conditions the transfer of hydrogens from G3P to fumarate, with attendant generation of high-energy phosphate. The phsophorylation process is more sensitive than the transhydrogenation process to inhibition by the detergent Triton X-100. The same is true with respect to sensitivity to sodium azide, carbonyl cyanide m-chlorophenylhydrazone and N,N'-dicyclohexylcarbodiimide. Such a preparation derived from cells with beta-galactoside permease can accumulate thiomethyl beta-D-galactoside anaerobically, and the accumulation can be stimulated twofold by adding G3P and fumarate. Mutants lacking the membrane-associated Mg2+-dependent adenosine triphosphatase cannot grow anaerobically on glycerol with fumarate as the hydrogen acceptor, although they can grow aerobically on glycerol alone.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenosine Triphosphatases / metabolism
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Anaerobiosis
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Azides / pharmacology
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Carbonyl Cyanide m-Chlorophenyl Hydrazone / pharmacology
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Cell Membrane / metabolism
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Dicyclohexylcarbodiimide / pharmacology
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Dihydroxyacetone Phosphate / metabolism
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Energy Metabolism
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Escherichia coli / enzymology
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Escherichia coli / metabolism*
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Fumarates / metabolism*
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Galactosides
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Glycerol / metabolism
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Glycerolphosphate Dehydrogenase / metabolism
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Glycerophosphates / metabolism*
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Hexosephosphates / metabolism
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Membrane Transport Proteins / metabolism
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Models, Biological
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Mutation
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Polyethylene Glycols / pharmacology
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Subcellular Fractions / metabolism
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Succinate Dehydrogenase / metabolism
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Succinates / metabolism
Substances
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Azides
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Fumarates
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Galactosides
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Glycerophosphates
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Hexosephosphates
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Membrane Transport Proteins
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Succinates
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Polyethylene Glycols
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Dicyclohexylcarbodiimide
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Carbonyl Cyanide m-Chlorophenyl Hydrazone
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Dihydroxyacetone Phosphate
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Glycerolphosphate Dehydrogenase
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Succinate Dehydrogenase
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Adenosine Triphosphatases
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Glycerol