Hormones play a central role in skin appearance and are implicated in skin aging. Recently, along with the remarkable increase in interest in natural products, the application of phytohormones in antiaging products has become very important. In this context, we developed date palm kernel extract. Date palm kernel is rich in phytohormones and we investigated the antiaging properties of date palm kernel in this in vivo study on wrinkles. Ten healthy women volunteers, between the ages of 46 and 58 years, applied the cream formula with 5% date palm kernel or placebo on the eye area twice a day for 5 weeks. The evaluation was made both clinically and by silicon replica analysis followed by statistical analysis using the Wilcoxon test. Silicon replica results showed that topical application of date palm kernel reduced the total surface of wrinkles by 27.6% (p = 0.038). Moreover, date palm kernel reduced the depth of wrinkles by 3.52% (p = 0.0231). These results are statistically significant and were clinically confirmed where visual improvement was seen in 60% of the volunteers treated. This in vivo study demonstrates that date palm kernel exhibits a significant antiwrinkle effect and is therefore of interest in antiaging skin care products.