Soluble IL-4 receptor improves the skin-graft-associated cytomegalovirus infection in thermally injured mice

Burns. 2003 Jun;29(4):315-21. doi: 10.1016/s0305-4179(02)00200-0.


The susceptibility of thermally injured mice (TI-mice) to murine cytomegalovirus (MCMV) infection is markedly influenced by burn-associated type 2 T cell responses, which are common with severe thermal injuries. In the present study, the effect of soluble IL-4 receptor (sIL-4R) on the skin-graft-associated MCMV infection was investigated. The marked growth of MCMV was demonstrated in the salivary glands of TI-mice grafted with MCMV seropositive [MCMV sero(+)] skin. However, the growth of MCMV was not demonstrated in the salivary glands of TI-mice grafted with MCMV sero(+) skin and treated with 50ng per mouse of sIL-4R. Compared with grafted normal mice, production of type 1 cytokines was markedly decreased when splenic T cells from TI-mice grafted with MCMV sero(+) skin were stimulated with anti-CD3 monoclonal antibody (mAb). The impaired type 1 cytokine production was recovered in cultures of splenic T cells from grafted TI-mice previously treated with sIL-4R. After grafting with MCMV sero(+) skin, the growth of MCMV was markedly inhibited in the salivary glands of severe combined immunodeficient (SCID) mice inoculated with T cells from TI-mice treated with sIL-4R. These results suggest that sIL-4R regulates the skin-graft-associated MCMV infection in TI-mice.

MeSH terms

  • Analysis of Variance
  • Animals
  • Burns / therapy
  • Burns / virology*
  • Cytomegalovirus Infections / prevention & control*
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Models, Animal
  • Receptors, Interleukin-4 / therapeutic use*
  • Salivary Glands / immunology
  • Salivary Glands / virology*
  • Skin Transplantation / immunology
  • T-Lymphocytes


  • Receptors, Interleukin-4