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. 2003 Jul 25;84(2):189-96.
doi: 10.1016/s0168-1605(02)00419-1.

Specific Identification of Certain Probiotic Lactobacillus Rhamnosus Strains With PCR Primers Based on Phage-Related Sequences

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Specific Identification of Certain Probiotic Lactobacillus Rhamnosus Strains With PCR Primers Based on Phage-Related Sequences

Kirsi Brandt et al. Int J Food Microbiol. .

Abstract

PCR primers derived from Lactobacillus rhamnosus phage Lc-Nu genome were used to screen the presence of phage-related sequences in Lb. rhamnosus strains. Several primer pairs derived from structural and replication gene regions of phage Lc-Nu amplified PCR products of expected sizes from bacterial strains revealing phage-related sequences in 10 of 11 Lb. rhamnosus strains. Strain-specific PCR primers for three probiotic Lb. rhamnosus strains were derived from these phage-related sequences for identification and detection purposes. Specificity of these primers was tested against 11 Lb. rhamnosus strains and over 40 other bacterial strains.

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