Specific identification of certain probiotic Lactobacillus rhamnosus strains with PCR primers based on phage-related sequences

Int J Food Microbiol. 2003 Jul 25;84(2):189-96. doi: 10.1016/s0168-1605(02)00419-1.


PCR primers derived from Lactobacillus rhamnosus phage Lc-Nu genome were used to screen the presence of phage-related sequences in Lb. rhamnosus strains. Several primer pairs derived from structural and replication gene regions of phage Lc-Nu amplified PCR products of expected sizes from bacterial strains revealing phage-related sequences in 10 of 11 Lb. rhamnosus strains. Strain-specific PCR primers for three probiotic Lb. rhamnosus strains were derived from these phage-related sequences for identification and detection purposes. Specificity of these primers was tested against 11 Lb. rhamnosus strains and over 40 other bacterial strains.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteriophages / genetics*
  • Base Sequence
  • DNA Primers
  • DNA, Bacterial / analysis
  • DNA, Bacterial / genetics
  • DNA, Viral / analysis
  • DNA, Viral / genetics
  • Lactobacillus / classification
  • Lactobacillus / isolation & purification
  • Lactobacillus / virology*
  • Polymerase Chain Reaction / methods*
  • Probiotics*
  • Sensitivity and Specificity
  • Species Specificity


  • DNA Primers
  • DNA, Bacterial
  • DNA, Viral