A new imprinted cluster on the human chromosome 7q21-q31, identified by human-mouse monochromosomal hybrids

Genomics. 2003 Jun;81(6):556-9. doi: 10.1016/s0888-7543(03)00052-1.


We have previously established a series of human monochromosomal hybrids containing a single human chromosome of defined parental origin as an in vitro resource for the investigation of human imprinted loci. Using the hybrids with a paternal or maternal human chromosome 7, we determined the allelic expression profiles of 76 ESTs mapped to the human chromosome 7q21-q31. Seven genes/transcripts, including PEG10 which has previously been reported to be imprinted, showed parent-of-origin-specific expression in monochromosomal hybrids. One of the 6 candidate genes/transcripts, i.e., DLX5 was confirmed to be imprinted in normal human lymphoblasts and brain tissues by a polymorphic analysis. Thus, an imprinted domain has been newly defined in the region of human chromosome 7q21-q31 using human-mouse monochromosomal hybrids.

MeSH terms

  • Animals
  • Apoptosis Regulatory Proteins
  • Brain / metabolism
  • Chimera
  • Chromosomes, Human, Pair 7 / genetics*
  • DNA-Binding Proteins
  • Expressed Sequence Tags
  • Genomic Imprinting / genetics*
  • Homeodomain Proteins / genetics*
  • Humans
  • Lymphocytes / metabolism
  • Mice / genetics
  • Multigene Family / genetics*
  • Proteins / genetics
  • RNA-Binding Proteins
  • Transcription Factors


  • Apoptosis Regulatory Proteins
  • DLX5 protein, human
  • DNA-Binding Proteins
  • Dlx5 protein, mouse
  • Homeodomain Proteins
  • PEG10 protein, human
  • Proteins
  • RNA-Binding Proteins
  • Transcription Factors