A pentatricopeptide repeat-containing gene that promotes the processing of aberrant atp6 RNA of cytoplasmic male-sterile rice

FEBS Lett. 2003 Jun 5;544(1-3):99-102. doi: 10.1016/s0014-5793(03)00480-0.

Abstract

A fertility restorer gene (Rf-1) of [ms-bo] cytoplasmic male sterility (CMS) in rice has been reported to be responsible for the processing of RNA of aberrant atp6 of mitochondria. We have carried out map-based cloning of the Rf-1 gene and found that a 4.7-kb genomic fragment of a restorer line promoted the processing of aberrant atp6 RNA when introduced into a CMS line. The genomic fragment contained a single open reading frame encoding 18 repeats of the 35 amino acid pentatricopeptide repeat (PPR) motif. The cloned PPR gene is a possible candidate of Rf-1. A non-restoring genotype was identified to have deletions within the coding region.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphatases / chemistry
  • Adenosine Triphosphatases / genetics*
  • Alleles
  • Amino Acid Motifs
  • Amino Acid Sequence
  • Blotting, Northern
  • Cell Nucleus / metabolism
  • Chromosome Mapping
  • Cloning, Molecular
  • Cytoplasm / metabolism*
  • Gene Deletion
  • Genetic Vectors
  • Genotype
  • Mitochondrial Proton-Translocating ATPases
  • Molecular Sequence Data
  • Open Reading Frames
  • Oryza / genetics*
  • Peptides / chemistry*
  • Phenotype
  • Recombinant Proteins / chemistry
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Homology, Amino Acid

Substances

  • Peptides
  • Recombinant Proteins
  • Adenosine Triphosphatases
  • Mitochondrial Proton-Translocating ATPases