Induction of MafBx and Murf ubiquitin ligase mRNAs in rat skeletal muscle after LPS injection

FEBS Lett. 2003 Jun 5;544(1-3):214-7. doi: 10.1016/s0014-5793(03)00505-2.

Abstract

MafBx and Murf are two new rat E3 ubiquitin ligases induced in muscle atrophy. Our goal was to investigate whether lipopolysaccharide (LPS) injection, a model of muscle catabolism, is associated with increased expression of MafBx and Murf. LPS (750 microg/100 g body weight) induces MafBx and Murf mRNA (respectively, 23-fold and 33-fold after 12 h; P<0.001). A transient induction of tumor necrosis factor-alpha mRNA (21-fold; P<0.001 at 3 h) and a decrease of insulin like growth factor-I mRNA (50%; P<0.001 at 6 h), two potential regulators of the ubiquitin-proteasome system were also demonstrated. In summary, MafBx and Murf mRNA are up-regulated in response to LPS and might play a role in the muscle proteolysis observed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Body Weight
  • Ligases / metabolism*
  • Lipopolysaccharides / metabolism*
  • Muscle Proteins / metabolism*
  • Muscle, Skeletal / drug effects
  • Muscle, Skeletal / metabolism*
  • Muscles / metabolism
  • RNA / metabolism
  • RNA, Messenger / metabolism
  • Rats
  • Rats, Wistar
  • Reverse Transcriptase Polymerase Chain Reaction
  • SKP Cullin F-Box Protein Ligases*
  • Time Factors
  • Tripartite Motif Proteins
  • Tumor Necrosis Factor-alpha / metabolism
  • Ubiquitin-Protein Ligases*
  • Up-Regulation

Substances

  • Lipopolysaccharides
  • Muscle Proteins
  • RNA, Messenger
  • Tripartite Motif Proteins
  • Tumor Necrosis Factor-alpha
  • RNA
  • FBXO32 protein, human
  • Fbxo32 protein, rat
  • SKP Cullin F-Box Protein Ligases
  • TRIM63 protein, human
  • Trim63 protein, rat
  • Ubiquitin-Protein Ligases
  • Ligases