Stretching and visualizing titin molecules: combining structure, dynamics and mechanics

J Muscle Res Cell Motil. 2002;23(5-6):499-511. doi: 10.1023/a:1023414624092.

Abstract

The details of the global and local structure and function of titin, a giant filamentous intrasarcomeric protein are largely undiscovered. Here we discuss a combination of bulk-solution and novel single-molecule techniques that may lend unique insights into titin's molecular dynamic, structural and mechanical characteristics.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Biomechanical Phenomena
  • Connectin
  • Dimerization
  • Elasticity
  • Fluorescence
  • Humans
  • Micromanipulation
  • Microscopy, Atomic Force
  • Microscopy, Confocal
  • Microspheres
  • Models, Molecular
  • Motion
  • Muscle Proteins / chemistry
  • Muscle Proteins / metabolism*
  • Muscle Proteins / ultrastructure*
  • Protein Binding
  • Protein Conformation
  • Protein Denaturation
  • Protein Isoforms / chemistry
  • Protein Isoforms / metabolism
  • Protein Isoforms / ultrastructure
  • Protein Kinases / chemistry
  • Protein Kinases / metabolism*
  • Protein Kinases / ultrastructure*
  • Rhodamines
  • Time Factors

Substances

  • Connectin
  • Muscle Proteins
  • Protein Isoforms
  • Rhodamines
  • TTN protein, human
  • Protein Kinases