Chronobiology of the proliferative events related to angiogenesis in mice liver regeneration after partial hepatectomy

Cell Biol Int. 2003;27(4):383-6. doi: 10.1016/s1065-6995(02)00289-5.


In liver regeneration the formation of new capillary blood vessels is a fundamental requirement for cellular proliferation. Vascular endothelial growth factor (VEGF) is involved in the events of angiogenesis, the mRNA of which is expressed in both hepatocytes and non-parenchymal cells. In this experimental design we try to establish if during liver regeneration in mouse, the expression of VEGF is produced before or after the hepatocytes proliferation. C3H/S adult male mice were divided in three groups in order to study: VEGF expression; S-phase index (SI); and mitotic activity (MA) of hepatocytes. The results that were analyzed by ANOVA, show that VEGF expression starts to increase 26 h after PH with a peak at 28 h. Furthermore, the DNA synthesis (DNAs) reaches maximal level 42 h after pH, meanwhile the MA of the hepatocytes shows an increase 8h after the DNAs peak. In conclusion, it could be argued that the chronobiology of the events related to liver regeneration in mice started with a release of VEGF by the hepatocytes, followed by its DNAs and mitosis.

MeSH terms

  • Animals
  • Cell Division / physiology
  • Chronobiology Phenomena / physiology*
  • Hepatectomy / methods
  • Hepatocytes / cytology*
  • Hepatocytes / physiology*
  • Liver / cytology
  • Liver / physiology
  • Liver / surgery
  • Liver Regeneration / physiology*
  • Male
  • Mice
  • Mice, Inbred C3H
  • Neovascularization, Physiologic / physiology*
  • Vascular Endothelial Growth Factor A / biosynthesis


  • Vascular Endothelial Growth Factor A