Regulation by nectin of the velocity of the formation of adherens junctions and tight junctions

Biochem Biophys Res Commun. 2003 Jun 20;306(1):104-9. doi: 10.1016/s0006-291x(03)00919-7.

Abstract

Cadherins are key Ca(2+)-dependent cell-cell adhesion molecules at adherens junctions (AJs) in fibroblasts and epithelial cells, whereas claudins are key Ca(2+)-independent cell-cell adhesion molecules at tight junctions (TJs) in epithelial cells. The formation and maintenance of TJs are dependent on the formation and maintenance of AJs. Nectins are Ca(2+)-independent immunoglobulin-like cell-cell adhesion molecules which comprise a family of four members, nectin-1, -2, -3, and -4, and are involved in the formation of AJs in cooperation with cadherins, and the subsequent formation of TJs. We show here that the velocity of the formation of the E-cadherin-based AJs is increased by overexpression of nectin-1 and is reduced by addition of the nectin-1 inhibitors to the medium in L cells stably expressing E-cadherin and Madin-Darby canine kidney cells. Moreover, the velocity of the formation of the claudin-based TJs is increased by overexpression of nectin-1 and is reduced by addition of the nectin-1 inhibitors to the medium in Madin-Darby canine kidney cells. These results indicate that nectins regulate the velocity of the formation of the E-cadherin-based AJs and the subsequent formation of the claudin-based TJs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adherens Junctions / physiology*
  • Animals
  • Cadherins / physiology
  • Cell Adhesion / physiology
  • Cell Adhesion Molecules / antagonists & inhibitors
  • Cell Adhesion Molecules / physiology*
  • Cell Line
  • Claudin-1
  • Dogs
  • Kinesin
  • Kinetics
  • L Cells
  • Membrane Proteins / physiology
  • Mice
  • Microfilament Proteins / physiology
  • Myosins
  • Nectins
  • Tight Junctions / physiology*

Substances

  • Afdn protein, mouse
  • Cadherins
  • Cell Adhesion Molecules
  • Claudin-1
  • Cldn1 protein, mouse
  • Membrane Proteins
  • Microfilament Proteins
  • Nectin1 protein, mouse
  • Nectins
  • afadin
  • Myosins
  • Kinesin