On the origins of cyanuric acid hydrolase: purification, substrates, and prevalence of AtzD from Pseudomonas sp. strain ADP

Appl Environ Microbiol. 2003 Jun;69(6):3653-7. doi: 10.1128/AEM.69.6.3653-3657.2003.

Abstract

Cyanuric acid hydrolase (AtzD) from Pseudomonas sp. strain ADP was purified to homogeneity. Of 22 cyclic amides and triazine compounds tested, only cyanuric acid and N-methylisocyanuric acid were substrates. Other cyclic amidases were found not to hydrolyze cyanuric acid. Ten bacteria that use cyanuric acid as a sole nitrogen source for growth were found to contain either atzD or trzD, but not both genes.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amidohydrolases / genetics
  • Amidohydrolases / isolation & purification*
  • Amidohydrolases / metabolism*
  • Bacterial Proteins / genetics
  • Bacterial Proteins / isolation & purification
  • Bacterial Proteins / metabolism
  • Evolution, Molecular*
  • Gram-Negative Bacteria / enzymology
  • Gram-Negative Bacteria / genetics
  • Herbicides / metabolism
  • Pseudomonas / enzymology*
  • Pseudomonas / genetics*
  • Substrate Specificity
  • Triazines / chemistry
  • Triazines / metabolism

Substances

  • Bacterial Proteins
  • Herbicides
  • Triazines
  • Amidohydrolases
  • cyanuric acid amidohydrolase
  • cyanuric acid