Human neutrophil antigen-4a gene frequencies in an Australian population, determined by a new polymerase chain reaction method using sequence-specific primers

H D Clague; Y L Fung; R M Minchinton

doi:10.1046/j.1365-3148.2003.00435.x

Human neutrophil antigen-4a gene frequencies in an Australian population, determined by a new polymerase chain reaction method using sequence-specific primers

Transfus Med. 2003 Jun;13(3):149-52. doi: 10.1046/j.1365-3148.2003.00435.x.

Authors

H D Clague¹, Y L Fung, R M Minchinton

Affiliation

¹ Queensland Institute of Medical Research, Australian Red Cross Blood Service, Brisbane, Queensland, Australia.

PMID: 12791082
DOI: 10.1046/j.1365-3148.2003.00435.x

Abstract

Human neutrophil antigen-4a (HNA-4a) is a high-frequency (99% in the USA) neutrophil antigen, which has recently been linked to a case of alloimmune neonatal neutropenia. We have devised a new polymerase chain reaction sequence-specific primer (PCR-SSP) method to assess HNA-4a genotype, and used it to determine the HNA-4a gene frequencies in an Australian population. The gene frequency was found to be 0.906, which is the same as in the American population. The PCR-SSP genotyping method perfectly correlates with serological phenotyping and is efficient for screening large numbers of samples.

Publication types

Research Support, Non-U.S. Gov't
Validation Study

MeSH terms

Australia / epidemiology
Base Sequence
DNA Primers
Gene Frequency
Genotype
Granulocytes
Humans
Isoantigens / genetics*
Molecular Epidemiology
Neutropenia / genetics
Polymerase Chain Reaction / methods*

Substances

DNA Primers
Isoantigens