Comparison of commercial DNA extraction kits for extraction of bacterial genomic DNA from whole-blood samples

J Clin Microbiol. 2003 Jun;41(6):2440-3. doi: 10.1128/jcm.41.6.2440-2443.2003.

Abstract

The demand for molecular diagnostic tests in medical microbiology has highlighted the need for efficient methods of DNA extraction. In addition, it is preferable for these methods to be automated. An example of such a requirement is for the confirmation of meningococcal disease where rapid, sensitive, and specific procedures are required for public health management purposes. Previous studies have shown that whole blood is the preferred method for the isolation of bacterial DNA in meningococcal disease, and in this study, we compare five commercially available kits for the extraction of bacterial genomic DNA from whole-blood samples. These include kits in a 96-well binding plate, 96-well filter plate, and metallic bead formats. The method for all five kits is described, and the sensitivity, specificity, ease of automation, and overall efficiency are determined.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blood / microbiology*
  • DNA, Bacterial / isolation & purification*
  • Genome, Bacterial*
  • Haemophilus influenzae / genetics
  • Haemophilus influenzae / isolation & purification
  • Humans
  • Neisseria meningitidis / genetics
  • Neisseria meningitidis / isolation & purification
  • Polymerase Chain Reaction / methods*
  • Reagent Kits, Diagnostic
  • Sensitivity and Specificity
  • Streptococcus pneumoniae / genetics
  • Streptococcus pneumoniae / isolation & purification

Substances

  • DNA, Bacterial
  • Reagent Kits, Diagnostic