Inhibitory effect of apelin-12 on nocturnal food intake in the rat

Nutr Neurosci. 2003 Jun;6(3):163-7. doi: 10.1080/1028415031000111273.


Apelin, the endogenous peptide ligand of the APJ receptor, is expressed in brain regions implicated in food and water intake. This study reports for the first time, the effect of apelin-12, one of the most potent apelin peptides, on spontaneous (nocturnal) feeding. Randomised intracerebroventricular injection of 1, 3 and 10 nmol apelin-12 or saline vehicle, 10 min prior to lights out, led to dose-dependent reductions in food intake 2-4 h after injection (n = 7; p < 0.05). This suggests that apelin-12 exerts a delayed inhibitory effect on nocturnal feeding. Relative to saline, no effect was observed on total 24-h food intake post injection. In contrast, day-time administration of 10 nmol apelin-12 to satiated rats stimulated feeding (n = 5-11; p < 0.05); lower doses had no effect. No changes in water intake were observed after apelin-12. These results suggest that apelin is involved in the central control of feeding.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apelin
  • Carrier Proteins / administration & dosage
  • Carrier Proteins / pharmacology*
  • Circadian Rhythm*
  • Dose-Response Relationship, Drug
  • Drinking / drug effects
  • Eating / drug effects*
  • Injections, Intraventricular
  • Intercellular Signaling Peptides and Proteins
  • Kinetics
  • Male
  • Peptide Fragments / administration & dosage
  • Peptide Fragments / pharmacology*
  • Rats
  • Rats, Sprague-Dawley
  • Satiation


  • Apelin
  • Apln protein, rat
  • Carrier Proteins
  • Intercellular Signaling Peptides and Proteins
  • Peptide Fragments