Effects of resveratrol and 4-hexylresorcinol on hydrogen peroxide-induced oxidative DNA damage in human lymphocytes

Free Radic Res. 2003 May;37(5):509-14. doi: 10.1080/1071576031000083099.

Abstract

The protective effects of resveratrol and 4-hexylresorcinol against oxidative DNA damage in human lymphocytes induced by hydrogen peroxide were investigated. Resveratrol and 4-hexylresorcinol showed no cytotoxicity to human lymphocytes at the tested concentration (10-100 microM). In addition, DNA damage in human lymphocytes induced by H2O2 was inhibited by resveratrol and 4-hexylresorcinol. Resveratrol and 4-hexylresorcinol at concentrations of 10-100 microM induced an increase in glutathione (GSH) levels in a concentration-dependent manner. Moreover, these two compounds also induced activity of glutathione peroxidase (GPX) and glutathione reductase (GR). The activity of glutathione-S-transferase (GST) in human lymphocytes was induced by resveratrol. Resveratrol and 4-hexylresorcinol inhibited the activity of catalase (CAT). These data indicate that the inhibition of resveratrol and 4-hexylresorcinol on oxidative DNA damage in human lymphocytes induced by H2O2 might be attributed to increase levels of GSH and modulation of antioxidant enzymes (GPX, GR and GST).

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antioxidants / pharmacology*
  • Catalase / drug effects
  • Catalase / metabolism
  • Cell Survival / drug effects
  • Comet Assay
  • DNA Damage*
  • Dose-Response Relationship, Drug
  • Glutathione / drug effects
  • Glutathione / metabolism
  • Glutathione Reductase / drug effects
  • Glutathione Reductase / metabolism
  • Glutathione Transferase / drug effects
  • Glutathione Transferase / metabolism
  • Hexylresorcinol / pharmacology*
  • Humans
  • Hydrogen Peroxide / administration & dosage*
  • Lymphocytes / drug effects*
  • Lymphocytes / metabolism
  • Oxidants / administration & dosage
  • Oxidation-Reduction
  • Peroxidases / drug effects
  • Peroxidases / metabolism
  • Resveratrol
  • Stilbenes / pharmacology*
  • Time Factors

Substances

  • Antioxidants
  • Oxidants
  • Stilbenes
  • Hydrogen Peroxide
  • Peroxidases
  • Catalase
  • Glutathione Reductase
  • Glutathione Transferase
  • Glutathione
  • Resveratrol
  • Hexylresorcinol