Human blood dendritic cells exhibit a distinct T-cell-stimulating mechanism and differentiation pattern

Scand J Immunol. 1992 Nov;36(5):689-96. doi: 10.1111/j.1365-3083.1992.tb03129.x.

Abstract

In this study, the mechanisms underlying stimulation of T-cell proliferation by human blood dendritic cells (BDC) and their differentiation have been defined with a panel of monoclonal antibodies (MoAbs). It was found that the MoAbs against LFA-1 (CD11a), CD11c, LFA-3 (CD58), ICAM-1 (CD54) or HLA-DR could significantly suppress T-cell proliferation in an allogeneic mixed lymphocyte reaction (P < 0.05), while being unable to inhibit clustering of BDC with T cells. Addition of anti-CD18 or CD45 MoAbs increased the size of clusters after 18 h of culture, but had no effect on the proliferation of T cells (P < 0.05). The suppressive effect of the MoAbs may be viewed not as an inhibition of contact between BDC and T cells, but rather as a blocking of co-stimulatory signals for T-cell activation, which are mediated by interaction of the adhesion molecules. After depleting the BDC preparations of monocytes, we used a double staining in FACS analysis to demonstrate that BDC do not express specific T (CD3), B (CD20 and CD21) and myeloid cell markers (CD11b, CD13 and CD14), but abundant class II antigens. This pattern remained unaltered after 8 days of culture in the presence of 100 U/ml GM-CSF, although a threefold increase of HLA-DQ and ICAM-1 molecules on the cultured cells was observed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal / immunology
  • Antigens, CD / physiology
  • Antigens, Surface / analysis
  • CD58 Antigens
  • Cell Adhesion Molecules / physiology
  • Cell Differentiation
  • Cells, Cultured
  • Dendritic Cells / physiology*
  • Humans
  • Intercellular Adhesion Molecule-1
  • Lymphocyte Activation*
  • Lymphocyte Culture Test, Mixed
  • Lymphocyte Function-Associated Antigen-1 / physiology
  • Membrane Glycoproteins / physiology
  • Phagocytosis
  • T-Lymphocytes / immunology*

Substances

  • Antibodies, Monoclonal
  • Antigens, CD
  • Antigens, Surface
  • CD58 Antigens
  • Cell Adhesion Molecules
  • Lymphocyte Function-Associated Antigen-1
  • Membrane Glycoproteins
  • Intercellular Adhesion Molecule-1