Differential subcellular localization of phosphorylated neurofilament and tau proteins in degenerating neurons of the human entorhinal cortex

Neuroreport. 2003 May 23;14(7):929-33. doi: 10.1097/01.wnr.0000072844.93264.31.


A panel of novel monoclonal antibodies was tested on the human entorhinal cortex for the recognition of age- and disease-related changes of neurofilament proteins (NF). Several antibodies identified phosphorylated NF-H subunit, which occurred preferentially in those aged between 60 and 80 years and were localized in degenerating neurons. Such neurons also contained neurofibrillary tangles, but neurofilament aggregates did not co-localize with tangles, nor did the quantity nor the number of NF-positive neurons correlate with the severity of Alzheimer's disease. This points to a susceptibility of NF in a subset of neurons for phosphorylation- and metabolically related morphological changes during neurodegeneration.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Aged, 80 and over
  • Entorhinal Cortex / chemistry
  • Entorhinal Cortex / metabolism*
  • Entorhinal Cortex / pathology
  • Female
  • Humans
  • Male
  • Middle Aged
  • Nerve Degeneration / metabolism*
  • Nerve Degeneration / pathology
  • Neurodegenerative Diseases / metabolism
  • Neurofilament Proteins / analysis
  • Neurofilament Proteins / metabolism*
  • Neurons / chemistry
  • Neurons / metabolism*
  • Neurons / pathology
  • Phosphorylation
  • Subcellular Fractions / chemistry
  • Subcellular Fractions / metabolism
  • tau Proteins / analysis
  • tau Proteins / metabolism*


  • Neurofilament Proteins
  • tau Proteins