Cloning of TRAP, a ligand for CD40 on human T cells

Eur J Immunol. 1992 Dec;22(12):3191-4. doi: 10.1002/eji.1830221226.


A cDNA clone, designated TRAP (TNF-related activation protein) was isolated from a collection of T cell activation genes. The polypeptide encoded by a mRNA of approx. 2.3 kb is 261 amino acids long with a calculated M(r) of 29.3 kDa. The structural features predict a type II transmembrane protein, but are also compatible with a secreted form. TRAP is highly similar to an identified murine CD40 ligand both at the cDNA (82.8% identity) and the protein (77.4% identity) levels, and related to tumor necrosis factor/lymphotoxin. Expressed in a murine myeloma, TRAP was identified as a ligand for CD40 by binding to a soluble CD40 construct. TRAP mRNA is expressed in a T cell-specific fashion with a maximum at 8 h after stimulation. The TRAP gene is located in the q26.3-q27.1 region of the X chromosome.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Antigens, CD / metabolism*
  • Antigens, Differentiation, B-Lymphocyte / metabolism*
  • Base Sequence
  • CD40 Antigens
  • CD40 Ligand
  • Chromosome Mapping
  • Cloning, Molecular*
  • Humans
  • Ligands
  • Membrane Glycoproteins / genetics*
  • Molecular Sequence Data
  • RNA, Messenger / analysis
  • Transfection
  • Tumor Necrosis Factor-alpha / genetics*


  • Antigens, CD
  • Antigens, Differentiation, B-Lymphocyte
  • CD40 Antigens
  • Ligands
  • Membrane Glycoproteins
  • RNA, Messenger
  • Tumor Necrosis Factor-alpha
  • CD40 Ligand

Associated data

  • GENBANK/S49007
  • GENBANK/X62960
  • GENBANK/X62961
  • GENBANK/X62962
  • GENBANK/X62963
  • GENBANK/X68550
  • GENBANK/Z14961
  • GENBANK/Z14962
  • GENBANK/Z14963
  • GENBANK/Z14964