Measles Virus V Protein Blocks Interferon (IFN)-alpha/beta but Not IFN-gamma Signaling by Inhibiting STAT1 and STAT2 Phosphorylation

FEBS Lett. 2003 Jun 19;545(2-3):177-82. doi: 10.1016/s0014-5793(03)00528-3.

Abstract

Measles virus (MV), a member of the family Paramyxoviridae, encodes C and V non-structural proteins. To clarify the functions of MV C and V proteins, HeLa cell lines constitutively expressing C or V protein were established. We found that expression of V protein inhibited interferon (IFN)-alpha/beta signaling but not IFN-gamma signaling. C protein had no inhibitory effect on IFN signaling in our experimental condition. Degradation of selective signal transducers and activators of transcription (STAT) proteins was not observed in HeLa cells expressing V protein. In contrast, tyrosine phosphorylation of both STAT1 and STAT2 was inhibited in these cells after IFN-beta stimulation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line
  • HeLa Cells
  • Humans
  • Interferon-alpha / antagonists & inhibitors*
  • Interferon-alpha / metabolism
  • Interferon-beta / antagonists & inhibitors*
  • Interferon-beta / metabolism
  • Interferon-gamma / metabolism*
  • Phosphoproteins / pharmacology*
  • Phosphorylation
  • Signal Transduction / drug effects*
  • Trans-Activators / genetics
  • Trans-Activators / metabolism*
  • Transcriptional Activation / drug effects*
  • Viral Proteins / pharmacology*

Substances

  • Interferon-alpha
  • Phosphoproteins
  • Trans-Activators
  • V protein, measles virus
  • Viral Proteins
  • Interferon-beta
  • Interferon-gamma