Efficacy of commercial and field-strain Mycobacterium paratuberculosis vaccinations with recombinant IL-12 in a bovine experimental infection model

Vaccine. 2003 Jul 4;21(23):3101-9. doi: 10.1016/s0264-410x(03)00261-5.


The efficacy of commercial (Strain 18) and field-isolate paratuberculosis vaccine preparations was investigated. The effect of prior exposure to Mycobacterium paratuberculosis and the adjuvant effect of rIL-12 on vaccine efficacy were also tested. Both Strain 18 and field-isolate vaccines induced strong local, systemic and enteric IFN-gamma responses. A significant reduction in mycobacterial colonization was observed when calves were vaccinated with the field-isolate prior to challenge, but not following vaccination with Strain 18 vaccine. Vaccination with rIL-12 prevented infection in some calves but its overall effect on IFN-gamma response and total mycobacterial load was not statistically significant. Efficacy of paratuberculosis vaccines may be enhanced if calves are vaccinated prior to M. paratuberculosis exposure with field-isolate vaccine instead of Strain 18 vaccine currently in use.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Adjuvants, Immunologic*
  • Animals
  • Antibodies, Bacterial / biosynthesis
  • Bacterial Vaccines / immunology*
  • Cattle
  • Cattle Diseases / immunology*
  • Cattle Diseases / prevention & control
  • Cells, Cultured
  • Cytokines / biosynthesis
  • Cytokines / genetics
  • Evaluation Studies as Topic
  • Feces / microbiology
  • Gene Expression
  • Humans
  • Interferon-gamma / biosynthesis
  • Interleukin-12 / immunology*
  • Male
  • Mycobacterium avium subsp. paratuberculosis / immunology*
  • Paratuberculosis / immunology*
  • Paratuberculosis / prevention & control
  • RNA, Messenger / biosynthesis
  • Recombinant Proteins / immunology
  • Reverse Transcriptase Polymerase Chain Reaction


  • Adjuvants, Immunologic
  • Antibodies, Bacterial
  • Bacterial Vaccines
  • Cytokines
  • RNA, Messenger
  • Recombinant Proteins
  • Interleukin-12
  • Interferon-gamma