Abstract
PsaF is a nuclear encoded gene for the subunit III of photosystem I. It is located at the lumenal side of the thylakoid membrane and interacts with plastocyanin. Starting from a low-level expression in the cotyledons of etiolated seedlings the gene is upregulated by light. Light can be replaced by Ca2+ or phosphoinositides like phorbol myristate acetate, an analogue of diacylglycerol. We tested the effects of these components on PsaF promoter-driven gene expression in roots and found that the PsaF promoter includes a positive regulatory region [-220/-179] activated by cytokinin and a negative regulatory region [-687/-221] activated by abscisic acid. In addition, the promoter is activated by Ca2+, mastoparan and phorbol myristate acetate which suggests a role for phospholipases and protein kinase C in PsaF gene expression.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Abscisic Acid / pharmacology
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Calcium / pharmacology
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Cytokinins / pharmacology
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Gene Expression Regulation, Plant* / drug effects
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Glucuronidase / genetics*
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Intercellular Signaling Peptides and Proteins
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Light
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Membrane Proteins / genetics*
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Nicotiana / drug effects
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Nicotiana / genetics*
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Peptides
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Photosynthetic Reaction Center Complex Proteins / genetics*
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Photosystem I Protein Complex*
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Plant Proteins*
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Plant Roots / drug effects
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Plant Roots / genetics*
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Plants, Genetically Modified
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Promoter Regions, Genetic / genetics*
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Protein Subunits / genetics
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Signal Transduction
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Spinacia oleracea / genetics*
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Tetradecanoylphorbol Acetate / pharmacology
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Wasp Venoms / pharmacology
Substances
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Cytokinins
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Intercellular Signaling Peptides and Proteins
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Membrane Proteins
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Peptides
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Photosynthetic Reaction Center Complex Proteins
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Photosystem I Protein Complex
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Plant Proteins
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Protein Subunits
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Wasp Venoms
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mastoparan
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Abscisic Acid
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Glucuronidase
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Tetradecanoylphorbol Acetate
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Calcium