Allofustis seminis gen. nov., sp. nov., a novel Gram-positive, catalase-negative, rod-shaped bacterium from pig semen

Int J Syst Evol Microbiol. 2003 May;53(Pt 3):811-814. doi: 10.1099/ijs.0.02484-0.


An unknown Gram-positive, catalase-negative, facultatively anaerobic, non-spore-forming, rod-shaped bacterium originating from semen of a pig was characterized using phenotypic, molecular chemical and molecular phylogenetic methods. Chemical studies revealed the presence of a directly cross-linked cell wall murein based on L-lysine and a DNA G + C content of 39 mol%. Comparative 16S rRNA gene sequencing showed that the unidentified rod-shaped organism formed a hitherto unknown subline related, albeit loosely, to Alkalibacterium olivapovliticus, Alloiococcus otitis, Dolosigranulum pigrum and related organisms, in the low-G + C-content Gram-positive bacteria. However, sequence divergence values of > 11% from these recognized taxa clearly indicated that the novel bacterium represents a separate genus. Based on phenotypic and phylogenetic considerations, it is proposed that the unknown bacterium from pig semen be classified as a new genus and species, Allofustis seminis gen. nov., sp. nov. The type strain is strain 01-570-1(T) (= CCUG 45438(T) = CIP 107425(T)).

MeSH terms

  • Animals
  • Bacterial Typing Techniques
  • Base Composition
  • Catalase / metabolism
  • Cell Wall / chemistry
  • DNA, Ribosomal / analysis
  • Gram-Positive Rods / chemistry
  • Gram-Positive Rods / classification*
  • Gram-Positive Rods / genetics
  • Gram-Positive Rods / isolation & purification*
  • Male
  • Molecular Sequence Data
  • Peptidoglycan / analysis
  • Phenotype
  • Phylogeny
  • RNA, Ribosomal, 16S / genetics
  • Semen / microbiology*
  • Sequence Analysis, DNA
  • Swine*


  • DNA, Ribosomal
  • Peptidoglycan
  • RNA, Ribosomal, 16S
  • Catalase

Associated data

  • GENBANK/AJ410303