Identification of a human src homology 2-containing protein-tyrosine-phosphatase: a putative homolog of Drosophila corkscrew

Proc Natl Acad Sci U S A. 1992 Dec 1;89(23):11239-43. doi: 10.1073/pnas.89.23.11239.


src homology 2 (SH2) domains direct binding to specific phosphotyrosyl proteins. Recently, SH2-containing protein-tyrosine-phosphatases (PTPs) were identified. Using degenerate oligonucleotides and the PCR, we have cloned a cDNA for an additional PTP, SH-PTP2, which contains two SH2 domains and is expressed ubiquitously. When expressed in Escherichia coli, SH-PTP2 displays tyrosine-specific phosphatase activity. Strong sequence similarity between SH-PTP2 and the Drosophila gene corkscrew (csw) and their similar patterns of expression suggest that SH-PTP2 is the human corkscrew homolog. Sequence comparisons between SH-PTP2, SH-PTP1, corkscrew, and other SH2-containing proteins suggest the existence of a subfamily of SH2 domains found specifically in PTPs, whereas comparison of the PTP domains of the SH2-containing PTPs with other tyrosine phosphatases suggests the existence of a subfamily of PTPs containing SH2 domains. Since corkscrew, a member of the terminal class signal transduction pathway, acts in concert with D-raf to positively transduce the signal generated by the receptor tyrosine kinase torso, these findings suggest several mechanisms by which SH-PTP2 may participate in mammalian signal transduction.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Brain / enzymology
  • Cloning, Molecular
  • Consensus Sequence
  • DNA / genetics
  • Gene Expression
  • Humans
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides / chemistry
  • Phosphotyrosine
  • Protein Tyrosine Phosphatases / genetics*
  • Protein Tyrosine Phosphatases / metabolism
  • RNA, Messenger / genetics
  • Rats
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Tyrosine / analogs & derivatives*
  • Tyrosine / metabolism


  • Oligodeoxyribonucleotides
  • RNA, Messenger
  • Phosphotyrosine
  • Tyrosine
  • DNA
  • Protein Tyrosine Phosphatases

Associated data

  • GENBANK/L03535