FY is an RNA 3' end-processing factor that interacts with FCA to control the Arabidopsis floral transition

Cell. 2003 Jun 13;113(6):777-87. doi: 10.1016/s0092-8674(03)00425-2.

Abstract

The nuclear RNA binding protein, FCA, promotes Arabidopsis reproductive development. FCA contains a WW protein interaction domain that is essential for FCA function. We have identified FY as a protein partner for this domain. FY belongs to a highly conserved group of eukaryotic proteins represented in Saccharomyces cerevisiae by the RNA 3' end-processing factor, Pfs2p. FY regulates RNA 3' end processing in Arabidopsis as evidenced through its role in FCA regulation. FCA expression is autoregulated through the use of different polyadenylation sites within the FCA pre-mRNA, and the FCA/FY interaction is required for efficient selection of the promoter-proximal polyadenylation site. The FCA/FY interaction is also required for the downregulation of the floral repressor FLC. We propose that FCA controls 3' end formation of specific transcripts and that in higher eukaryotes, proteins homologous to FY may have evolved as sites of association for regulators of RNA 3' end processing.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence / genetics
  • Arabidopsis / genetics*
  • Arabidopsis / metabolism
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism*
  • Base Sequence / genetics
  • DNA, Complementary / analysis
  • DNA, Complementary / genetics
  • Flowers / genetics*
  • Flowers / metabolism
  • MADS Domain Proteins / genetics
  • MADS Domain Proteins / metabolism
  • Molecular Sequence Data
  • Mutation / genetics
  • Plant Proteins / genetics
  • Plant Proteins / isolation & purification*
  • Polyadenylation / genetics
  • Protein Structure, Tertiary / genetics
  • RNA 3' End Processing / genetics*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism*
  • RNA-Binding Proteins / genetics
  • RNA-Binding Proteins / isolation & purification*
  • RNA-Binding Proteins / metabolism*
  • Repressor Proteins / genetics
  • Reproduction / genetics
  • Saccharomyces cerevisiae Proteins / genetics
  • Saccharomyces cerevisiae Proteins / metabolism
  • mRNA Cleavage and Polyadenylation Factors / genetics
  • mRNA Cleavage and Polyadenylation Factors / metabolism

Substances

  • Arabidopsis Proteins
  • DNA, Complementary
  • FLF protein, Arabidopsis
  • Flowering time control protein FCA, Arabidopsis
  • MADS Domain Proteins
  • Pfs2 protein, S cerevisiae
  • Plant Proteins
  • RNA, Messenger
  • RNA-Binding Proteins
  • Repressor Proteins
  • Saccharomyces cerevisiae Proteins
  • mRNA Cleavage and Polyadenylation Factors