Feasibility of atmospheric pressure desorption/ionization on silicon mass spectrometry in analysis of drugs

K Huikko; P Östman; C Sauber; F Mandel; K Grigoras; S Franssila; T Kotiaho; R Kostiainen

doi:10.1002/rcm.1051

Feasibility of atmospheric pressure desorption/ionization on silicon mass spectrometry in analysis of drugs

Rapid Commun Mass Spectrom. 2003;17(12):1339-1343. doi: 10.1002/rcm.1051.

Authors

K Huikko¹, P Östman¹, C Sauber², F Mandel², K Grigoras³, S Franssila³, T Kotiaho¹, R Kostiainen^{1

4}

Affiliations

¹ Viikki Drug Discovery Technology Center, Department of Pharmacy, P.O. Box 56, FIN-00014 University of Helsinki, Helsinki, Finland.
² Agilent Technologies, Deutschland GmbH, Hewlett-Packard-Straße 8, 76337 Waldbronn, Germany.
³ Microelectronics Centre, Helsinki University of Technology, P.O. Box 3000, FIN-02015 HUT, Finland.
⁴ Division of Pharmaceutical Chemistry, Department of Pharmacy, P.O. Box 56, FIN-00014 University of Helsinki, Helsinki, Finland.

PMID: 12811757
DOI: 10.1002/rcm.1051

Abstract

The feasibility of atmospheric pressure desorption/ionization on silicon mass spectrometry (AP-DIOS-MS) for drug analysis was investigated. It was observed that only compounds with relative high proton affinity are efficiently ionized under AP-DIOS conditions. The limits of detection (LODs) achieved in MS mode with midazolam, propranolol, and angiotensin II were 80 fmol, 20 pmol, and 1 pmol, respectively. In MS/MS mode the LODs for midazolam and propranolol were 10 fmol and 5 pmol, respectively. The good linearity (r(2) > 0.991), linear dynamic range of 3 orders of magnitude, and reasonable repeatability showed that the method is suitable for quantitative analysis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acetaminophen / analogs & derivatives*
Acetaminophen / analysis
Acetaminophen / chemistry
Air Ionization
Atmospheric Pressure*
Carboxylic Acids / analysis
Carboxylic Acids / chemistry
Ketoprofen / analysis
Ketoprofen / chemistry
Mass Spectrometry / methods*
Midazolam / analysis
Midazolam / chemistry
Molecular Structure
Naphthaleneacetic Acids / analysis
Naphthaleneacetic Acids / chemistry
Naphthalenes / analysis
Naphthalenes / chemistry
Naphthoquinones / analysis
Naphthoquinones / chemistry
Pharmaceutical Preparations / analysis*
Pharmaceutical Preparations / chemistry
Propranolol / analysis
Propranolol / chemistry
Silicon / chemistry*
Testosterone / analysis
Testosterone / chemistry

Substances

Carboxylic Acids
Naphthaleneacetic Acids
Naphthalenes
Naphthoquinones
Pharmaceutical Preparations
1-naphthoic acid
Acetaminophen
Testosterone
2-naphthylacetic acid
acetaminophen glucuronide
Ketoprofen
Propranolol
Midazolam
1,4-naphthoquinone
Silicon