Structural and functional properties of IL-4delta2, an alternative splice variant of human IL-4

J Proteome Res. May-Jun 2003;2(3):273-81. doi: 10.1021/pr025586y.

Abstract

Structural and functional properties of recombinant IL-4delta2, a naturally occurring splice variant of human IL-4 with a deletion of the loop region 22-37, have been analyzed. IL-4delta2 has alpha-helical structure and most likely preserves the "up-up-down-down" topology typical of the four-helix-bundle cytokines. IL-4delta2 interacts specifically with the alpha chain of IL-4R and competes effectively with IL-4 for the common binding sites. Thus, IL-4delta2 may act as a regulator of the cytokine net, being the natural antagonist of IL-4.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alternative Splicing*
  • Cell Division / physiology
  • Circular Dichroism
  • Cloning, Molecular
  • Cystine / metabolism
  • Interleukin-4 / genetics*
  • Interleukin-4 / metabolism
  • Ligands
  • Protein Isoforms / genetics*
  • Protein Isoforms / metabolism
  • Protein Structure, Tertiary
  • Spectroscopy, Fourier Transform Infrared
  • Thymus Gland / metabolism

Substances

  • Ligands
  • Protein Isoforms
  • Interleukin-4
  • Cystine