Bioelectronic DNA detection of human papillomaviruses using eSensor: a model system for detection of multiple pathogens

BMC Infect Dis. 2003 Jun 19;3:12. doi: 10.1186/1471-2334-3-12.

Abstract

Background: We used human papillomaviruses (HPV) as a model system to evaluate the utility of a nucleic acid, hybridization-based bioelectronic DNA detection platform (eSensor) in identifying multiple pathogens.

Methods: Two chips were spotted with capture probes consisting of DNA oligonucleotide sequences specific for HPV types. Electrically conductive signal probes were synthesized to be complementary to a distinct region of the amplified HPV target DNA. A portion of the HPV L1 region that was amplified by using consensus primers served as target DNA. The amplified target was mixed with a cocktail of signal probes and added to a cartridge containing a DNA chip to allow for hybridization with complementary capture probes.

Results: Two bioelectric chips were designed and successfully detected 86% of the HPV types contained in clinical samples.

Conclusions: This model system demonstrates the potential of the eSensor platform for rapid and integrated detection of multiple pathogens.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Biosensing Techniques / methods
  • DNA Primers
  • DNA Probes, HPV
  • Humans
  • Microbiological Techniques / methods*
  • Oligonucleotide Array Sequence Analysis / methods*
  • Papillomaviridae / isolation & purification*

Substances

  • DNA Primers
  • DNA Probes, HPV