Multiphoton endoscopy

Opt Lett. 2003 Jun 1;28(11):902-4. doi: 10.1364/ol.28.000902.


Despite widespread use of multiphoton fluorescence microscopy, development of endoscopes for nonlinear optical imaging has been stymied by the degradation of ultrashort excitation pulses that occurs within optical fiber as a result of the combined effects of group-velocity dispersion and self-phase modulation. We introduce microendoscopes (350-1000 microm in diameter) based on gradient-index microlenses that effectively eliminate self-phase modulation within the endoscope. Laser-scanning multiphoton fluorescence endoscopy exhibits micrometer-scale resolution. We used multiphoton endoscopes to image fluorescently labeled neurons and dendrites.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain / cytology
  • Brain / ultrastructure
  • Endoscopy*
  • Equipment Design
  • Microscopy, Fluorescence
  • Models, Theoretical*
  • Neurons / ultrastructure
  • Photons*
  • Songbirds