Studies on the substrate specificity of Escherichia coli galactokinase

Org Lett. 2003 Jun 26;5(13):2223-6. doi: 10.1021/ol034642d.


In vitro glycorandomization (IVG) technology is dependent upon the ability to rapidly synthesize sugar phosphates. Compared with chemical synthesis, enzymatic (kinase) routes to sugar phosphates would be attractive for this application. This work focuses upon the development of a high-throughput colorimetric galactokinase (GalK) assay and its application toward probing the substrate specificity and kinetic parameters of Escherichia coli GalK. The demonstrated dinitrosalicylic assay should also be generally applicable to a variety of sugar-processing enzymes. [reaction: see text]

MeSH terms

  • Colorimetry
  • Escherichia coli / enzymology*
  • Galactokinase / chemistry*
  • Galactokinase / metabolism*
  • Galactose / analogs & derivatives
  • Galactose / metabolism
  • Glycosides / chemistry
  • Kinetics
  • Salicylates / chemistry
  • Substrate Specificity


  • Glycosides
  • Salicylates
  • Galactokinase
  • Galactose