Determination of methylparaben, propylparaben, triamcinolone acetonide and its degradation product in a topical cream by RP-HPLC

Anal Bioanal Chem. 2003 Jun;376(4):440-3. doi: 10.1007/s00216-003-1756-x. Epub 2003 Feb 18.


A novel reversed-phase high-performance liquid chromatographic (RP-HPLC) method was developed and validated for the determination of active component triamcinolone acetonide, its degradation product triamcinolone (occurring in formulation after long-term stability tests) and two preservatives presented in the cream-methylparaben and propylparaben, using hydrocortisone as an internal standard. The chromatographic separation was performed on a Supelco Discovery C18 column; the mobile phase for separation of all compounds consists of a mixture of acetonitrile and water (40:60 v/v). The analysis time was less than 9 min, at a flow rate of 0.6 mL min(-1) and detection at 240 nm. The method was found to be applicable for routine analysis (stability tests, homogeneity) in the pharmaceutical product topical cream Triamcinolon cream 0.1%.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Topical
  • Anti-Inflammatory Agents / analysis*
  • Anti-Inflammatory Agents / chemistry
  • Chromatography, High Pressure Liquid
  • Drug Stability
  • Glucocorticoids
  • Ointments / chemistry
  • Parabens / analysis
  • Preservatives, Pharmaceutical / analysis*
  • Quality Control
  • Triamcinolone / analysis*
  • Triamcinolone Acetonide / analysis*
  • Triamcinolone Acetonide / chemistry


  • Anti-Inflammatory Agents
  • Glucocorticoids
  • Ointments
  • Parabens
  • Preservatives, Pharmaceutical
  • Triamcinolone
  • methylparaben
  • Triamcinolone Acetonide
  • propylparaben