Linomide improves the effect of interstitial laser thermotherapy in a rat liver tumour model

Anticancer Res. Mar-Apr 2003;23(2B):1257-63.

Abstract

Aim: The aim of this study was to investigate if Linomide affects growth and spread of a rat liver tumour when given alone and in combination with interstitial laser thermotherapy (ILT).

Materials and methods: Experiments were performed in Wistar rats using a dimethyl-hydrazine-induced adenocarcinoma implanted into the left lateral lobe of the liver. The rats were randomised to one of the following groups: a) ILT and Linomide, b) ILT only, c) sham ILT, d) Linomide only, or e) control. ILT was intentionally suboptimal. Linomide (100 mg/kg/day) was given in the drinking water from the start of treatment for five days. ED1, ED2 macrophages and v Willebrand (factor VIII) were determined by an immunohistochemical technique.

Results: Linomide reduced viable liver tumour volume both when it was given alone (p < 0.01) and when combined with ILT (p < 0.05), whereas it lowered intraperitoneal spread in ILT-treated rats alone. Six days after ILT, there was a reduction in the number of newly-recruited macrophages and blood vessels in the viable tumour tissue in rats receiving Linomide.

Conclusion: Linomide reduced the growth of an adenocarcinoma transplanted into rat liver, when given alone or combined with laser thermotherapy and reduced the spread of tumour in laser-treated rats. The effects of Linomide in laser-treated rats appeared, at least in part, to be due to a reduction in newly-formed vessels, which might have been secondary to a reduced number of tumour-associated macrophages.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenocarcinoma / blood supply
  • Adenocarcinoma / drug therapy
  • Adenocarcinoma / secondary*
  • Adenocarcinoma / therapy*
  • Adjuvants, Immunologic / pharmacology
  • Adjuvants, Immunologic / therapeutic use
  • Angiogenesis Inhibitors / pharmacology
  • Angiogenesis Inhibitors / therapeutic use
  • Animals
  • Antineoplastic Agents / pharmacology
  • Antineoplastic Agents / therapeutic use*
  • Colonic Neoplasms / pathology
  • Combined Modality Therapy
  • Drug Screening Assays, Antitumor
  • Hydroxyquinolines / pharmacology
  • Hydroxyquinolines / therapeutic use*
  • Hyperthermia, Induced*
  • Lasers
  • Liver Neoplasms, Experimental / blood supply
  • Liver Neoplasms, Experimental / drug therapy
  • Liver Neoplasms, Experimental / secondary*
  • Liver Neoplasms, Experimental / therapy
  • Macrophages / pathology
  • Male
  • Random Allocation
  • Rats
  • Rats, Wistar

Substances

  • Adjuvants, Immunologic
  • Angiogenesis Inhibitors
  • Antineoplastic Agents
  • Hydroxyquinolines
  • roquinimex