Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 1 (3), 160-3

A Sensitive, Quantitative Assay for Measurement of Allele-Specific Transcripts Differing by a Single Nucleotide

Affiliations

A Sensitive, Quantitative Assay for Measurement of Allele-Specific Transcripts Differing by a Single Nucleotide

J Singer-Sam et al. PCR Methods Appl.

Abstract

We have found that the single nucleotide primer extension assay, a PCR-based assay currently used qualitatively to measure allelic differences in DNA, can be used quantitatively to measure allele-specific transcripts differing by only a single nucleotide. We show that total RNA containing the Pgk-1a transcript can be specifically detected even in a 1000-fold excess of RNA containing the Pgk-1b transcript.

Similar articles

See all similar articles

Cited by 31 articles

See all "Cited by" articles

Publication types

LinkOut - more resources

Feedback