A sensitive, quantitative assay for measurement of allele-specific transcripts differing by a single nucleotide

PCR Methods Appl. 1992 Feb;1(3):160-3. doi: 10.1101/gr.1.3.160.


We have found that the single nucleotide primer extension assay, a PCR-based assay currently used qualitatively to measure allelic differences in DNA, can be used quantitatively to measure allele-specific transcripts differing by only a single nucleotide. We show that total RNA containing the Pgk-1a transcript can be specifically detected even in a 1000-fold excess of RNA containing the Pgk-1b transcript.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alleles
  • Animals
  • Base Sequence
  • DNA / genetics*
  • Mice
  • Molecular Sequence Data
  • Polymerase Chain Reaction / methods*
  • Polymerase Chain Reaction / statistics & numerical data
  • RNA / genetics
  • Sensitivity and Specificity
  • Transcription, Genetic


  • RNA
  • DNA