Intracellular release of nitric oxide by NCX 972, an NO-releasing metronidazole, enhances in vitro killing of Entamoeba histolytica

Antimicrob Agents Chemother. 2003 Jul;47(7):2303-6. doi: 10.1128/AAC.47.7.2303-2306.2003.


The activity of NCX 972, a new molecule obtained by adding a nitric oxide (NO) moiety to metronidazole, was tested against six isolates of Entamoeba histolytica in xenic cultures. NCX 972 released NO into trophozoite cells and enhanced killing of amoebas in a dose- and time-dependent manner compared to metronidazole.

MeSH terms

  • Animals
  • Antitrichomonal Agents / chemistry
  • Antitrichomonal Agents / pharmacology*
  • Dose-Response Relationship, Drug
  • Drug Design
  • Entamoeba histolytica / drug effects*
  • In Vitro Techniques
  • Metronidazole / chemistry
  • Metronidazole / pharmacology*
  • Nitric Oxide / chemistry*


  • Antitrichomonal Agents
  • Metronidazole
  • Nitric Oxide