Intracellular release of nitric oxide by NCX 972, an NO-releasing metronidazole, enhances in vitro killing of Entamoeba histolytica

Anna Sannella; Luigi Gradoni; Tiziana Persichini; Ennio Ongini; Giorgio Venturini; Marco Colasanti

doi:10.1128/AAC.47.7.2303-2306.2003

Intracellular release of nitric oxide by NCX 972, an NO-releasing metronidazole, enhances in vitro killing of Entamoeba histolytica

Antimicrob Agents Chemother. 2003 Jul;47(7):2303-6. doi: 10.1128/AAC.47.7.2303-2306.2003.

Authors

Anna Sannella¹, Luigi Gradoni, Tiziana Persichini, Ennio Ongini, Giorgio Venturini, Marco Colasanti

Affiliation

¹ Laboratory of Parasitology, Istituto Superiore di Sanità, University of Rome "Roma Tre," Milan, Italy.

Abstract

The activity of NCX 972, a new molecule obtained by adding a nitric oxide (NO) moiety to metronidazole, was tested against six isolates of Entamoeba histolytica in xenic cultures. NCX 972 released NO into trophozoite cells and enhanced killing of amoebas in a dose- and time-dependent manner compared to metronidazole.

MeSH terms

Animals
Antitrichomonal Agents / chemistry
Antitrichomonal Agents / pharmacology*
Dose-Response Relationship, Drug
Drug Design
Entamoeba histolytica / drug effects*
In Vitro Techniques
Metronidazole / chemistry
Metronidazole / pharmacology*
Nitric Oxide / chemistry*

Substances

Antitrichomonal Agents
Metronidazole
Nitric Oxide