Hodgkin's lymphoma cell lines express a fusion protein encoded by intergenically spliced mRNA for the multilectin receptor DEC-205 (CD205) and a novel C-type lectin receptor DCL-1

J Biol Chem. 2003 Sep 5;278(36):34035-41. doi: 10.1074/jbc.M303112200. Epub 2003 Jun 24.


Classic Hodgkin's lymphoma (HL) tissue contains a small population of morphologically distinct malignant cells called Hodgkin and Reed-Sternberg (HRS) cells, associated with the development of HL. Using 3'-rapid amplification of cDNA ends (RACE) we identified an alternative mRNA for the DEC-205 multilectin receptor in the HRS cell line L428. Sequence analysis revealed that the mRNA encodes a fusion protein between DEC-205 and a novel C-type lectin DCL-1. Although the 7.5-kb DEC-205 and 4.2-kb DCL-1 mRNA were expressed independently in myeloid and B lymphoid cell lines, the DEC-205/DCL-1 fusion mRNA (9.5 kb) predominated in the HRS cell lines (L428, KM-H2, and HDLM-2). The DEC-205 and DCL-1 genes comprising 35 and 6 exons, respectively, are juxtaposed on chromosome band 2q24 and separated by only 5.4 kb. We determined the DCL-1 transcription initiation site within the intervening sequence by 5'-RACE, confirming that DCL-1 is an independent gene. Two DEC-205/DCL-1 fusion mRNA variants may result from cotranscription of DEC-205 and DCL-1, followed by splicing DEC-205 exon 35 or 34-35 along with DCL-1 exon 1. The resulting reading frames encode the DEC-205 ectodomain plus the DCL-1 ectodomain, the transmembrane, and the cytoplasmic domain. Using DCL-1 cytoplasmic domain-specific polyclonal and DEC-205 monoclonal antibodies for immunoprecipitation/Western blot analysis, we showed that the fusion mRNA is translated into a DEC-205/DCL-1 fusion protein, expressed in the HRS cell lines. These results imply an unusual transcriptional control mechanism in HRS cells, which cotranscribe an mRNA containing DEC-205 and DCL-1 prior to generating the intergenically spliced mRNA to produce a DEC-205/DCL-1 fusion protein.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alternative Splicing
  • Amino Acid Sequence
  • Antigens, CD / chemistry*
  • Base Sequence
  • Blotting, Northern
  • Blotting, Western
  • Cell Line
  • Chromosomes / metabolism
  • Cytoplasm / metabolism
  • DNA, Complementary / metabolism
  • Enzyme-Linked Immunosorbent Assay
  • Exons
  • HL-60 Cells
  • Hodgkin Disease / metabolism*
  • Humans
  • Jurkat Cells
  • Lectins / metabolism
  • Lectins, C-Type / chemistry*
  • Minor Histocompatibility Antigens
  • Models, Genetic
  • Molecular Sequence Data
  • Oncogene Proteins, Fusion / chemistry*
  • Oncogene Proteins, Fusion / metabolism*
  • Precipitin Tests
  • Protein Structure, Tertiary
  • RNA Splicing
  • RNA, Messenger / metabolism
  • Receptors, Cell Surface / chemistry*
  • Receptors, Cell Surface / metabolism*
  • Recombinant Fusion Proteins / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Time Factors
  • Transcription, Genetic
  • Tumor Cells, Cultured


  • Antigens, CD
  • CD302 protein, human
  • DEC-205 receptor
  • DNA, Complementary
  • LY75-CD302 readthrough, human
  • Lectins
  • Lectins, C-Type
  • Minor Histocompatibility Antigens
  • Oncogene Proteins, Fusion
  • RNA, Messenger
  • Receptors, Cell Surface
  • Recombinant Fusion Proteins

Associated data

  • GENBANK/AY184222
  • GENBANK/AY314006
  • GENBANK/AY314007