Induction of the blood-brain barrier specific HT7 and neurothelin epitopes in endothelial cells of the chick chorioallantoic vessels by a soluble factor derived from astrocytes

Brain Res Dev Brain Res. 1992 Dec 18;70(2):207-11. doi: 10.1016/0165-3806(92)90199-7.


Some blood-brain barrier properties of microvascular endothelial cells have been shown to be inducible by astrocytes. We tested the hypothesis that this cellular interaction is mediated by a soluble factor(s). Chick chorioallantoic vessels in ovo were constantly exposed to astrocyte-conditioned medium. We found that endothelial cells exposed to astrocyte-derived factors, but not to glioma- or endothelial cell-derived factors, expressed the HT7 antigen and neurothelin, two specific markers of the blood-brain barrier phenotype. These results indicate that a soluble factor(s) secreted by astrocytes is capable to induce specific blood-brain barrier properties in endothelial cells of non-neural origin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, CD*
  • Antigens, Neoplasm*
  • Antigens, Surface*
  • Astrocytes / physiology*
  • Avian Proteins*
  • Basigin
  • Blood Proteins*
  • Blood-Brain Barrier*
  • Chick Embryo
  • Endothelium, Vascular / immunology*
  • Epitopes / physiology
  • Extraembryonic Membranes / blood supply*
  • Gene Expression
  • Membrane Glycoproteins / metabolism*
  • Rats
  • Rats, Sprague-Dawley


  • Antigens, CD
  • Antigens, Neoplasm
  • Antigens, Surface
  • Avian Proteins
  • Blood Proteins
  • Bsg protein, Gallus gallus
  • Bsg protein, rat
  • Epitopes
  • Membrane Glycoproteins
  • Basigin