The prognosis in spindle-cell sarcoma depends on the expression of cyclin-dependent kinase inhibitor p27(Kip1) and cyclin E

Cancer Sci. 2003 May;94(5):412-7. doi: 10.1111/j.1349-7006.2003.tb01456.x.


The aim of the present study was to examine the prognostic significance of p27(Kip1) and cyclin E expression in patients with spindle-cell soft tissue sarcomas. In 46 cases of spindle-cell sarcoma including 17 pre-operative biopsy materials, the expression of p27(Kip1) and cyclin E was immunohistochemically examined. The expression of p27(Kip1) decreased in the nuclei of metastatic primary tumor cells (stage IV), whereas the expression of cyclin E increased in those lesions. On univariate analysis, when the expression of p27(Kip1) and cyclin E was analyzed together, patients with spindle-cell sarcoma exhibiting low expression of p27(Kip1) and high expression of cyclin E showed lower distant-metastasis-free survival (DMFS) and overall survival (OS) than those with other combinations of the two parameters (both P < 0.0001). Multivariate analysis revealed that patients with low p27(Kip1) and high cyclin E expression also showed a decrease in DMFS (P = 0.0007, relative risk = 21.3) and OS (P = 0.005, relative risk = 20.8). These results suggest that the combination analysis of p27(Kip1) and cyclin E expression even in biopsy specimens allows the prediction of the clinical behavior of spindle-cell sarcoma.

MeSH terms

  • Cell Cycle Proteins / metabolism*
  • Cyclin E / metabolism*
  • Cyclin-Dependent Kinase Inhibitor p27
  • Female
  • Humans
  • Immunoenzyme Techniques
  • Male
  • Middle Aged
  • Neoplasm Staging
  • Prognosis
  • Sarcoma / metabolism*
  • Sarcoma / mortality
  • Sarcoma / pathology
  • Soft Tissue Neoplasms / metabolism*
  • Soft Tissue Neoplasms / mortality
  • Soft Tissue Neoplasms / pathology
  • Survival Rate
  • Tumor Suppressor Proteins / metabolism*


  • Cell Cycle Proteins
  • Cyclin E
  • Tumor Suppressor Proteins
  • Cyclin-Dependent Kinase Inhibitor p27