PhiC31 integrase-mediated nonviral genetic correction of junctional epidermolysis bullosa

Hum Gene Ther. 2003 Jun 10;14(9):923-8. doi: 10.1089/104303403765701204.

Abstract

Patients afflicted with severe laminin 5-deficient junctional epidermolysis bullosa (JEB) often die in infancy with massive cutaneous blistering. Prior approaches to genetically correct this disorder have relied on stable integration of wild-type LAMB3 sequences, using retroviral vectors. To develop a nonviral approach to JEB gene therapy, we used the phiC31 integrase, which mediates unidirectional genomic integration of plasmids containing a specific attB site. An attB-containing laminin 5 beta3 expression plasmid was integrated into the genomes of primary keratinocytes from four unrelated, genetically characterized JEB patients. phiC31 integrase supported genomic integration into epidermal progenitor cells. Regeneration of human skin on immunedeficient mice, using these cells, produced human skin tissue with restored laminin 5 expression. Furthermore, corrected JEB tissue restored hemidesmosome formation and abolished histologic evidence of subepidermal blistering. These findings provide an approach to durable nonviral correction of JEB.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Adhesion Molecules / genetics*
  • Cell Adhesion Molecules / immunology
  • Cell Adhesion Molecules / metabolism
  • Cells, Cultured
  • Epidermolysis Bullosa, Junctional / metabolism
  • Epidermolysis Bullosa, Junctional / therapy*
  • Gene Expression
  • Genetic Therapy*
  • Genetic Vectors
  • Humans
  • Integrases / genetics*
  • Keratinocytes / physiology
  • Keratinocytes / ultrastructure
  • Mice
  • Mice, SCID
  • Plasmids
  • Regeneration
  • Skin / anatomy & histology

Substances

  • Cell Adhesion Molecules
  • kalinin
  • Integrases