Antitumor activities of heteropolysaccharides of Poria cocos mycelia from different strains and culture media

Carbohydr Res. 2003 Jul 4;338(14):1517-21. doi: 10.1016/s0008-6215(03)00198-8.


Ten water-soluble heteropolysaccharide fractions were isolated from Poria cocos mycelia cultured from one wild and one cultivated strain in two identical culture media differing only in one component: either corn steep liquor or bran extract. The chemical compositions, including monosaccharide profile, protein content, and molecular mass M(w) of the mycelial polysaccharides were determined. Both the in vitro and in vivo antitumor activities of the heteropolysaccharides were evaluated and compared. The heteropolysaccharides from Poria cocos mycelia cultured with the wild strain in a medium containing corn steep liquor exhibited the highest antitumor activities against Sarcoma 180 in vivo.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antineoplastic Agents / analysis
  • Antineoplastic Agents / chemistry
  • Antineoplastic Agents / pharmacology*
  • Cell Line, Tumor / drug effects
  • Chlorocebus aethiops
  • Culture Media / pharmacology
  • Dose-Response Relationship, Drug
  • HL-60 Cells / drug effects
  • Humans
  • Mice
  • Mice, Inbred BALB C
  • Mycelium / chemistry*
  • Mycelium / drug effects
  • Polyporales / chemistry*
  • Polyporales / drug effects
  • Polysaccharides / analysis
  • Polysaccharides / chemistry
  • Polysaccharides / pharmacology*
  • Sarcoma 180 / drug therapy
  • Vero Cells / drug effects


  • Antineoplastic Agents
  • Culture Media
  • Polysaccharides