Ionic liquids in embalming and tissue preservation. Can traditional formalin-fixation be replaced safely?

Acta Histochem. 2003;105(2):135-42. doi: 10.1078/0065-1281-00707.

Abstract

Ionic liquids (ILs) can be used for embalming and tissue preservation. ILs does not cause tissue damage and the tissue colour remains unaltered after treatment. Microscopical morphology of tissues fixed in ILs is of better quality than that of tissues fixed in formalin. Tissue preservation depends on the type of ILs. Best results were obtained with 1-methyl-3-octyloxymethylimidazolium tetrafluoroborate, the density of which resembles that of water. The salt is nonvaporous and when used as a formalin substitute, it eliminates health hazards in the pathological laboratory.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacteria / drug effects
  • Bacteria / genetics
  • Borates / chemical synthesis
  • Borates / chemistry*
  • Borates / pharmacology
  • Breast / anatomy & histology
  • Embalming / methods*
  • Formaldehyde / chemistry*
  • Humans
  • Imidazoles / chemical synthesis
  • Imidazoles / chemistry*
  • Imidazoles / pharmacology
  • Ions / chemical synthesis
  • Ions / chemistry*
  • Ions / pharmacology
  • Liver / anatomy & histology
  • Microbial Sensitivity Tests
  • Models, Chemical
  • Muscle, Skeletal / anatomy & histology
  • Rats
  • Salts / chemistry
  • Solutions / chemistry
  • Solvents / chemical synthesis
  • Solvents / chemistry*
  • Solvents / pharmacology
  • Tissue Fixation / methods
  • Tissue Preservation / methods*

Substances

  • 1-methyl-3-octyloxymethylimidazolium tetrafluoroborate
  • Borates
  • Imidazoles
  • Ions
  • Salts
  • Solutions
  • Solvents
  • Formaldehyde