Crystallin gene expression in the process of lentoidogenesis in cultures of chicken lens epithelial cells

Exp Eye Res. 1992 Dec;55(6):879-87. doi: 10.1016/0014-4835(92)90014-j.

Abstract

One alpha B- and three different beta-crystallin cDNA clones were isolated from a chicken lens cDNA library by using anti-crystallin antibodies. The sequence of alpha B-crystallin cDNA showed more than 70% homology with exons of alpha B-crystallin genes of the human and hamster. Two beta-crystallin cDNAs showed almost identical sequences with previously reported chicken beta B1- and beta A3/A1-crystallin genes. The remainder showed 80% homology of sequence with bovine beta B2-crystalline cDNA. Using these newly cloned cDNAs, in addition to cDNAs of alpha A- and delta-crystallin, we examined the expression pattern of these crystallins in the process of lentoidogenesis of cultured lens epithelial cells of the chicken. All crystallins except beta-crystallins were expressed through the period of cell culture, but three beta-crystallins were expressed only after the confluent stage. These results suggest that: (1) alpha A-, alpha B- and delta-crystallin cDNAs can be used to detect differentiation of the lens epithelial cell; and (2) beta-crystallin cDNAs are superior in the detection of chicken lens fibre differentiation in vitro to delta-crystallin cDNA, which is ectopically expressed by various non-lenticular tissues.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies, Monoclonal / genetics
  • Base Sequence*
  • Blotting, Northern
  • Cells, Cultured
  • Cross Reactions
  • Crystallins / genetics*
  • Epithelium
  • Gene Expression
  • Lens, Crystalline / chemistry*
  • Molecular Sequence Data
  • RNA / analysis

Substances

  • Antibodies, Monoclonal
  • Crystallins
  • RNA

Associated data

  • GENBANK/S50854
  • GENBANK/S50855
  • GENBANK/S52930
  • GENBANK/S53164
  • GENBANK/S90657
  • GENBANK/S90659
  • GENBANK/S90758
  • GENBANK/S90759
  • GENBANK/X67411
  • GENBANK/X67412