High-resolution structure of human apo dipeptidyl peptidase IV/CD26 and its complex with 1-[([2-[(5-iodopyridin-2-yl)amino]-ethyl]amino)-acetyl]-2-cyano-(S)-pyrrolidine

Acta Crystallogr D Biol Crystallogr. 2003 Jul;59(Pt 7):1206-12. doi: 10.1107/s0907444903010059. Epub 2003 Jun 27.

Abstract

Dipeptidyl peptidase IV is a multifunctional type II transmembrane serine protease glycoprotein. The high-resolution crystal structure of the homodimeric human apo dipeptidyl peptidase IV has been determined at 1.9 A resolution. In addition, the structure of the binary complex with 1-[([2-[(5-iodopyridin-2-yl)amino]-ethyl]amino)-acetyl]-2-cyano-(S)-pyrrolidine has been solved, revealing the nature of the covalent interaction with the active-site serine.

MeSH terms

  • Aminopyridines / chemistry*
  • Apoenzymes / chemistry
  • Binding Sites
  • Crystallization
  • Crystallography, X-Ray
  • Dipeptidyl Peptidase 4 / chemistry*
  • Dipeptidyl Peptidase 4 / isolation & purification
  • Enzyme Inhibitors / chemistry
  • Humans
  • Molecular Structure
  • Protein Binding
  • Protein Conformation
  • Pyrrolidines / chemistry*

Substances

  • 1-(((2-((5-iodopyridin-2-yl)amino)ethyl)amino)acetyl)-2-cyanopyrrolidine
  • Aminopyridines
  • Apoenzymes
  • Enzyme Inhibitors
  • Pyrrolidines
  • Dipeptidyl Peptidase 4