Ventral neurons in the anterior nerve cord of amphioxus larvae. I. An inventory of cell types and synaptic patterns

Abstract

Serial sections were used to map the ventrally positioned neurons of the anterior nerve cord of a 12.5-day amphioxus larva from the infundibular region to the end of somite 2. Synaptic patterns reveal five categories of descending pathways, four of which are associated with the ventral compartment (VC) motoneurons responsible for escape swimming. 1) Pre-, para-, and postinfundibular (tegmental) neurons with large varicosities and mixed vesicle populations provide both synaptic and paracrine input to various components of the tegmental neuropile and primary motor center. Four categories of these neurons are distinguished on the basis of their vesicles. 2) Multiple anterior sensory pathways converge on the large paired neurons (LPNs) located near the junction of somites 1 and 2. LPN synaptic output is almost exclusively contralateral. This, together with the evidence for cross-innervation between the third pair of LPNs, is consistent with the latter acting as locomotory pacemakers. 3) Axons from several classes of tegmental neurons converge in the paraxial region on each side of the cord where they form distinct tracts, the upper paraxial bundles. The right bundle is larger than the left, which suggests a role during early development when myotome contractions are biased to one side. 4) Fibers in the ventral tracts from ipsilateral projection neurons, sensory neurons, and additional ascending fibers synapse repeatedly with VC motoneurons. This may be how the overall level of excitation of the latter is controlled so as to modulate their response to pacemaker input. The fifth pathway consists of fibers involved in controlling the dorsal compartment (DC) motoneurons responsible for slow swimming, which are largely isolated from inputs to the VC locomotory system. The ventral neurons of the primary motor center form a more or less continuous file on either side of the floor plate, with certain cell types showing a tendency to cluster. There are, however, few obvious patterns of the kind expected if development were controlled by a rigid, lineage-based mechanism. The evolutionary implications of the involvement of a midbrain-level pacemaker in controlling larval swimming in amphioxus is discussed.