Unique scanning capabilities of a new hybrid linear ion trap mass spectrometer (Q TRAP) used for high sensitivity proteomics applications

Proteomics. 2003 Jun;3(6):859-69. doi: 10.1002/pmic.200300415.

Abstract

The unique scanning capabilities of a hybrid linear ion trap (Q TRAP) mass spectrometer are described with an emphasis on proteomics applications. The combination of the very selective triple quadrupole based tandem mass spectrometry (MS/MS) scans with the very sensitive ion trap product ion scans allows rapid identification of peptides at low concentrations derived from post-translationally modified proteins on chromatographic time scales. The Q TRAP instrument also offers the opportunity to conduct a variety of ion processing steps prior to performing a mass scan. For example, the enhancement of the multiple-charge ion contents of the ion trap can be performed resulting in a survey mass spectrum dominated by double- and triple-charge peptides. This facilitates the identification of relevant biological species in both separated and unseparated peptide mixtures for further MS/MS experiments.

MeSH terms

  • Animals
  • Cattle
  • Chromatography, High Pressure Liquid
  • Mass Spectrometry / instrumentation*
  • Phosphopeptides / analysis*
  • Proteome*
  • Sensitivity and Specificity
  • Serum Albumin, Bovine / analysis

Substances

  • Phosphopeptides
  • Proteome
  • Serum Albumin, Bovine