Novel taurine-containing uridine derivatives and mitochondrial human diseases

T Suzuki; T Suzuki; T Wada; K Saigo; K Watanabe

doi:10.1093/nass/1.1.257

Novel taurine-containing uridine derivatives and mitochondrial human diseases

Nucleic Acids Res Suppl. 2001:(1):257-8. doi: 10.1093/nass/1.1.257.

Authors

T Suzuki¹, T Suzuki, T Wada, K Saigo, K Watanabe

Affiliation

¹ Dept of Integrated Biosciences, Graduate School of Frontier Sciences, Dept of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba Prefecture, 277-8562, Japan.

PMID: 12836362
DOI: 10.1093/nass/1.1.257

Abstract

Two novel modified uridines were identified from mammalian mitochondrial (mt) tRNAs. Mass spectrometric analysis revealed that they are modified uridines possessing a sulfonic acid group derived from taurine; 5-taurinomethyl-uridine from mt tRNAs for Trp and Leu(UUR), and 5-taurinomethyl-2-thiouridine from mt tRNAs for Lys, Gln and Glu. We have found lack of modification of these taurine-containing uridines in mutant mt tRNAs for Leu(UUR) and Lys from pathogenic cells of mitochondrial encephalomyopathies, MELAS and MERRF, respectively.

MeSH terms

Humans
Mass Spectrometry
Mitochondrial Encephalomyopathies / genetics*
RNA / chemistry*
RNA, Mitochondrial
RNA, Transfer / chemistry*
Taurine / chemistry*
Uridine / analogs & derivatives*

Substances

RNA, Mitochondrial
Taurine
RNA
RNA, Transfer
Uridine