Structure-based experimental confirmation of biochemical function to a methyltransferase, MJ0882, from hyperthermophile Methanococcus jannaschii

J Struct Funct Genomics. 2002;2(3):121-7. doi: 10.1023/a:1021279113558.

Abstract

We have determined the three-dimensional (3-D) structure of protein MJ0882, which derives from a hypothetical open reading frame in the genome of the hyperthermophile Methanococcus jannaschii. The 3-D fold of MJ0882 at 1.8 A highly resembles that of a methyltransferase, despite limited sequence similarity to any confirmed methyltransferase. The structure has an S-adenosylmethionine (AdoMet) binding pocket surrounded by motifs with similarities to those commonly found among AdoMet binding proteins. Preliminary biochemical experiments show that MJ0882 specifically binds to AdoMet, which is the essential co-factor for methyltransferases.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Motifs
  • Amino Acid Sequence
  • Bacterial Proteins / chemistry*
  • Bacterial Proteins / physiology
  • Crystallography, X-Ray
  • Methanococcus / enzymology*
  • Methanococcus / genetics
  • Models, Molecular
  • Molecular Sequence Data
  • Mutagenesis, Site-Directed
  • NAD / metabolism
  • Open Reading Frames
  • Protein Binding
  • Protein Conformation
  • Recombinant Fusion Proteins / chemistry
  • Recombinant Fusion Proteins / metabolism
  • S-Adenosylhomocysteine / metabolism
  • S-Adenosylmethionine / metabolism
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Site-Specific DNA-Methyltransferase (Adenine-Specific) / chemistry*
  • Site-Specific DNA-Methyltransferase (Adenine-Specific) / physiology
  • Structure-Activity Relationship

Substances

  • Bacterial Proteins
  • Recombinant Fusion Proteins
  • NAD
  • S-Adenosylmethionine
  • S-Adenosylhomocysteine
  • Site-Specific DNA-Methyltransferase (Adenine-Specific)

Associated data

  • PDB/1DUS