Two-photon Imaging to a Depth of 1000 Microm in Living Brains by Use of a Ti:Al2O3 Regenerative Amplifier

Opt Lett. 2003 Jun 15;28(12):1022-4. doi: 10.1364/ol.28.001022.

Abstract

It is shown that two-photon fluorescence images can be obtained throughout almost the entire gray matter of the mouse neocortex by using optically amplified femtosecond pulses. The achieved imaging depth approaches the theoretical limit set by excitation of out-of-focus fluorescence.

MeSH terms

  • Aluminum Oxide
  • Animals
  • Blood Vessels / anatomy & histology
  • Brain / anatomy & histology*
  • Cerebral Cortex / anatomy & histology
  • Cerebrovascular Circulation
  • Dextrans
  • Fluorescein-5-isothiocyanate / analogs & derivatives*
  • Green Fluorescent Proteins
  • Imaging, Three-Dimensional
  • Indicators and Reagents
  • Luminescent Proteins
  • Mice
  • Mice, Transgenic
  • Microscopy, Fluorescence*
  • Periaqueductal Gray / anatomy & histology
  • Photons*
  • Titanium

Substances

  • Dextrans
  • Indicators and Reagents
  • Luminescent Proteins
  • fluorescein isothiocyanate dextran
  • Green Fluorescent Proteins
  • Titanium
  • Fluorescein-5-isothiocyanate
  • Aluminum Oxide