DP epitope mapping by using T-cell clones

Hum Immunol. 1992 Oct;35(2):100-8. doi: 10.1016/0198-8859(92)90017-h.


To determine whether a correlation exists between the genomic HLA class II DP DNA polymorphism and cell surface expression and to detect the DP epitopes responsible for alloreactivity, anti-DP T-cell clones were generated against new PLT blank RFLP DPa and DPb-defined specificities. The clones were tested on the 10th IHWS B-LCLs and on local panel cells. Oligotyping of the tested cells made it possible to (a) correlate the DPa specificity with the DPB1*0402 specificity and (b) split DPb into DPB1*1001 and DPB1*1401. By comparing DNA sequences of the second exon to panel reactivity, the epitopes responsible for DPB1*1001 and 1401 were defined and attributed to beta-chain residues contributing to peptide selection inside the HLA groove. However, DNA sequences could not explain anti-DPa allospecificity, indicating that another structure not yet definable may be involved.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Antibodies, Monoclonal
  • B-Lymphocytes / immunology
  • Clone Cells
  • Epitopes / genetics*
  • HLA-DP Antigens / biosynthesis
  • HLA-DP Antigens / genetics*
  • Humans
  • Lymphocyte Culture Test, Mixed
  • Molecular Sequence Data
  • Peptide Mapping / methods*
  • Polymorphism, Genetic
  • Polymorphism, Restriction Fragment Length
  • Sequence Homology, Amino Acid
  • T-Lymphocytes / immunology


  • Antibodies, Monoclonal
  • Epitopes
  • HLA-DP Antigens